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New methods for preparation and analysis of S-adenosyl-(5′)-3-methylthiopropylamine

✍ Scribed by Vincenzo Zappia; Patrizia Galletti; Adriana Oliva; Antonio De Santis

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
502 KB
Volume
79
Category
Article
ISSN
0003-2697

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✦ Synopsis

In order to separate S-adenosyl-(5')-3-methylthiopropylamine (decarboxylated AdoMet) from its biological precursor, S-adenosylmethionine (AdoMet), two procedures have been devised. The first method involves two sequential chromatographic steps on Dowex 50, H+ form, and Dowex 1, OH-form, respectively. An alternate procedure takes advantage of the unusual heat stability of decarboxylated AdoMet, as compared to its precursor, and permits the purification of the sulfonium compound by single-step chromatography. The method utilizes an acid hydrolysis, at lOO"C, of a mixture of the two compounds, resulting in a selective cleavage of AdoMet into homoserine and 5'-methyhhioadenosine:

A single-step chromatography on Dowex 50, Na+ form, permits the separation of the unmodified S-adenosyl-(5')-3-methylthiopropylamine from the hydrolytic products of AdoMet. The yield is quantitative, and the procedure is simple and highly reproducible. 535

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