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New Method for Manipulation, Maintenance, and Cloning of Single Mammalian Cells in vitro

✍ Scribed by Aronson, M.; Kessel, R. W. I.

Book ID
121165984
Publisher
American Association for the Advancement of Science
Year
1960
Tongue
English
Weight
559 KB
Volume
131
Category
Article
ISSN
0036-8075

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## Abstract Chinese hamster cells (line CHO) were cloned in suspension with a carboxymethylcellulose‐containing medium, and the colonies were counted with an electronic particle counter after enzymatically degrading the carboxymethylcellulose with cellulase. Survival of x‐irradiated cells was deter