New method for assay of tyrosine transaminase

✍ Scribed by Bernard Schepartz

• Publisher: Elsevier Science
• Year: 1969
• Tongue: English
• Weight: 338 KB
• Volume: 30
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(69)90139-0

✦ Synopsis

2-oxoglutarate aminotransferase, EC 2.6.1.5) activity has been assayed by a number of methods in recent years, including the photometric procedures of Briggs (1) (modified by Canellakis and Cohen (2))) Lin et al. (3), and Diamondstone (4)) the isotopic methods of Weinstein et al. ( 5)) Gabay and George (6), Wurtman and Larin ( 7)) and Litwack and Squires ( 8)) as well as new isotopic and photometric methods recently reported in preliminary form by Fellman et al. ( 9). The multiplicity of methods reflects not so much the dissatisfaction of later investigators with already available methods as it does varying requirements for sensitivity, specificity, speed, handling of multiple samples, interference by other substances present, etc. In our own case, an investigation of the effects of certain phenolic inhibitors on tyrosine transaminase (to be published elsewhere) led to the establishment of yet another photometric method. Our starting point was the discovery of a specific color reaction between arylpyruvates and phenazine methosulfate (10).