๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Assay of tyrosine transaminase activity by conversion of p-hydroxyphenylpyruvate to p-hydroxybenzaldehyde

โœ Scribed by Thomas I. Diamondstone


Publisher
Elsevier Science
Year
1966
Tongue
English
Weight
407 KB
Volume
16
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis


In the course of investigat'ing some kinetic properties of relatively crude preparations of tyrosine transaminase (L-tyrosine:2-oxoglutarate aminotransferase, EC 2.6.1.5), it became necessary to use rather low concentrations of enzyme. The concentrations involved were so low that the modified Briggs assay? (1, 2) was no longer sensitive enough to measure activities accurately and conveniently under those conditions.


๐Ÿ“œ SIMILAR VOLUMES


Inhibition of adenylate kinase by P1,P5-
โœ William N. Valentine; Donald E. Paglia; Misae Nakatani; Richard A. Brockway ๐Ÿ“‚ Article ๐Ÿ“… 1989 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 191 KB

P1,P5-di(adenosine 5')pentaphosphate (Ap5A) is an excellent inhibitor of human hemolysate adenylate kinase at concentrations near 2 microM and above. At ten times this concentration and in hemolysate enzyme assays under conditions described in this paper it appears not to alter reaction data in the