✦ LIBER ✦

New ligands for boronate affinity chromatography

✍ Scribed by Xiao-Chuan Liu; William H. Scouten

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 871 KB
Volume: 687
Category: Article
ISSN: 1873-3778
DOI: 10.1016/0021-9673(94)00863-9

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

New aliphatic boronate ligands for affin

New aliphatic boronate ligands for affinity chromatography

✍ Vladimir Adamek; Xiao-Chuan Liu; Yu An Zhang; Katerina Adamkova; William H. Scou 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 739 KB

Photocleavable Biotinylated Ligands for

Photocleavable Biotinylated Ligands for Affinity Chromatography

✍ C. Thiele; F. Fahrenholz 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 731 KB

Two derivatives of the peptide hormone cholecystokinin (CCK-8s) containing a photocleavable o-nitrobenzylester group have been synthesized. One analog, [3H]4-(biotin-epsilon-Ahx-oxymethyl)-3-nitrobenzoyl-Gly-Orn (propionyl)-epsilon-Ahx-CCK-8s ([3H]BANA-CCK-8s), was biotinylated, while the other, 4-a

Isolation of 3,4-dihydroxyphenylalanine-

Isolation of 3,4-dihydroxyphenylalanine-containing proteins using boronate affinity chromatography

✍ Clifford J. Hawkins; Martin F. Lavin; David L. Parry; Ian L. Ross 📂 Article 📅 1986 🏛 Elsevier Science 🌐 English ⚖ 325 KB

A rapid procedure for the isolation of 3,4-dihydroxyphenylalanine-containing proteins has been developed in which the protein is selectively bound to a m-phenylboronate agarose column, and eluted with 1.0 M ammonium acetate, pH 3.0. The method is based on the affinity of boronates for diols includin

Specific Ligands for the Affinity Chroma

Specific Ligands for the Affinity Chromatography of Cholinergic Proteins

✍ Gaetano Riggio; Wolfgang Herbert Hopff; Alfred André Hofmann; Peter Gaudenz Wase 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 German ⚖ 571 KB

## Abstract Affinity chromatography of proteins requires a ligand covalently bound to a solid support separated by a spacer of sufficient length. In the specific case of acetyl‐cholinesterase we have reduced the conventional spacer synthesis from five to three steps. For affinity chromatography of

New α-amino phenylalanine tetrazole liga

New α-amino phenylalanine tetrazole ligand for immobilized metal affinity chromatography of proteins

✍ Genhu Lei; Liting Liu; Xiaohu Xiong; Yinmao Wei; Xiaohui Zheng 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 698 KB

## Abstract A new chelating compound has been developed for use in the immobilized metal affinity chromatographic (IMAC) separation of proteins. The bidentate ligand, α‐amino phenylalanine tetrazole, **4**, was synthesized __via__ a five‐step synthesis from __N__‐fluorenylmethoxycarbonyl phenylalan

Affinity chromatography without immobili

Affinity chromatography without immobilized ligands; A new method for studying macromolecular interactions using high-performance liquid chromatography

✍ Shigeru Endo; Hidechika Hayashi; Akiyoshi Wada 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 635 KB

A new method for studying macromolecular interactions was devised. The principle is based on affinity chromatography with a mobile zone of affinity ligand instead of a column with immobilized ligand. In this method, the difference in migration velocities between the moving zone of affinity ligand an