New insights into the dynamics of sinusoidal endothelial fenestrae in liver sinusoidal endothelial cells
β Scribed by Hiroaki Yokomori
- Book ID
- 106239017
- Publisher
- Springer
- Year
- 2008
- Tongue
- English
- Weight
- 254 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0918-4287
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π SIMILAR VOLUMES
Targeting of cells to specific tissues is critical for cell therapy. To study endothelial cell targeting, we isolated mouse liver sinusoidal endothelial cells (LSEC) and examined cell biodistributions in animals. To identify transplanted LSEC in tissues, we labeled cells metabolically with DiI-conju
that infiltrating T cells are activated and produce inflamma-CD4 / T lymphocytes have been identified as being retory lymphokines. 3,4 In experimental autoimmune hepatitis, sponsible for organ damage in the murine model of ex-T-cell-mediated liver damage could be demonstrated. 5 Howperimental liver
2 from mouse livers and cocultured them with primitive Once separated, LEC-1 and LEC-2 were cultured on collagen-coated hematopoietic cells from day-2, post-5-fluorouracil-treated 24-well plates in IMDM supplemented with 20% fetal calf serum mice. Our results show that LEC-1, but not LEC-2, supports