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Extramedullary hematopoiesis in the adult mouse liver is associated with specific hepatic sinusoidal endothelial cells

✍ Scribed by J E Cardier; E Barberá-Guillem

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 810 KB
Volume: 26
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.510260122

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✦ Synopsis

2 from mouse livers and cocultured them with primitive Once separated, LEC-1 and LEC-2 were cultured on collagen-coated hematopoietic cells from day-2, post-5-fluorouracil-treated 24-well plates in IMDM supplemented with 20% fetal calf serum mice. Our results show that LEC-1, but not LEC-2, supports (FCS) (BioWhittaker) and antibiotics. Cultures were maintained at in vitro long-term hematopoiesis of primitive hematopoietic 37ЊC in a humidified atmosphere of 5% CO 2 .

progenitors. These results are consistent with the premise Cell Cloning. The LEC-1 and LEC-2 were cloned by limiting dilu-

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