๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

New enhancers for the chemiluminescent peroxidase catalysed chemiluminescent oxidation of pyrogallol and purpurogallin

โœ Scribed by Nozaki, Osamu ;Ji, Xiaoying ;Kricka, Larry J.

Publisher
John Wiley and Sons
Year
1995
Weight
426 KB
Volume
10
Category
Article
ISSN
0884-3996

No coin nor oath required. For personal study only.

โœฆ Synopsis

The effects of various boronate compounds, 4-biphenylboronic acid, 4-bromobenzeneboronic acid, trans-4-(3-propionic acid)phenylboronic acid and 4-iodophenylboronic acid, on the horseradish peroxidase (HRP) catalysed chemiluminescent oxidation of pyrogallol and purpurogallin by peroxide were investigated. frans-4-(3-Propionic acid)phenylboronic acid produced a 13.7-fold enhancement in the peak light emission from the chemiluminescent HRP catalysed pyrogallol reaction (detection limit for HRP < 1.25fmol). At low enhancer concentration a single peak of light emission was observed and as the enhancer concentration increased the time to peak light emission became progressively longer. The chemiluminescence showed t w o peaks at higher concentrations (>54.3 pmol/L) and the individual peak times depended upon the concentration of the enhancer. All of the boronates enhanced peak light emission in the chemiluminescent HRP catalysed purpurogallin reaction. 4-Biphenylboronic acid was the most effective and it enhanced peak light emission 314-fold. The practical detection limit for HRP (Type VIA) using this enhancer was 4.18pmol (peak emission a t 20 minutes). This compound also enhanced peak light emission 232-fold from a chemiluminescent HRP-purpurogallin reaction in which molecular oxygen replaced peroxide as the oxidant.

๐Ÿ“œ SIMILAR VOLUMES

4-phenylylboronic acid: A new type of en
4-phenylylboronic acid: A new type of enhancer for the horseradish peroxidase catalysed chemiluminescent oxidation of luminol
โœ Kricka, Larry J. ;Ji, Xiaoying ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› John Wiley and Sons โš– 347 KB

4-Phenylylboronic acid enhances the light emission from the horseradish peroxidase catalysed oxidation of luminol by hydrogen peroxide. Optimization studies showed that the greatest enhancement was obtained using micromolar concentrations of the new enhancer. The largest degree of enhancement was fo

Enhanced chemiluminescence reaction appl
Enhanced chemiluminescence reaction applied to the study of horseradish peroxidase stability in the course ofp-iodophenol oxidation
โœ Kapeluich, Yuri L. ;Rubtsova, Maya Yu. ;Egorov, Alexey M. ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons โš– 124 KB ๐Ÿ‘ 2 views

The enhanced chemiluminescence reaction (ECL) was applied to the study of horseradish peroxidase (HRP) inactivation during the oxidation of p-iodophenol. Enzyme inactivation was shown to be the main reason for light decay in the course of the reaction. No individual effect of luminol and p-iodopheno

New luminol chemiluminescence reaction u
New luminol chemiluminescence reaction using diperiodatoargentate as oxidate for the determination of amikacin sulfate
โœ Chunyan Yang; Zhujun Zhang; Jinli Wang ๐Ÿ“‚ Article ๐Ÿ“… 2009 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 555 KB

## Abstract A new chemiluminescence (CL) reaction between luminol and diperiodatoargentate {K~2~ [Ag (H~2~IO~6~) (OH) ~2~]} was observed in alkaline medium. The CL intensity could be greatly enhanced by amikacin sulfate. Therefore a new CL method for the determination of amikacin sulfate was built

CCD camera imaging for the chemiluminesc
CCD camera imaging for the chemiluminescent detection of enzymes using new ultrasensitive reagents
โœ Akhavan-Tafti, Hashem ;Schaap, A. Paul ;Arghavani, Zahara ;Desilva, Renuka ;Eick ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› John Wiley and Sons โš– 703 KB

## Abstract We have designed and constructed an inexpensive imaging system based on charge coupled device (CCD) technology and utilized it to demonstrate the sensitivity and rapid detection possible with Lumigenยฎ chemiluminescent reagents. We also report the development of two new chemiluminescent