The lac operon shows anomalous expression in Proteus mirabilis: the maximal induced level is 10% or less of that in E. coli, while repression reduces this by a factor of only 2-5. We have sought to determine whether this effect relates in any way to CRP-mediated activation of expression, by comparin
New Controlling Element in the Lac Operon of E. coli
โ Scribed by IPPEN, KARIN; MILLER, JEFFREY H.; SCAIFE, JOHN; BECKWITH, JONATHAN
- Book ID
- 109662103
- Publisher
- Nature Publishing Group
- Year
- 1968
- Tongue
- English
- Weight
- 323 KB
- Volume
- 217
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/217825a0
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๐ SIMILAR VOLUMES
The lac operon introduced into Proteus mirabilis shows two anomalies of expression: the maximal induced level is reduced by about an order of magnitude, and the basal level becomes about 100 times higher than in E. coli, as a result of which the induction ratio appears very small, around 2-5 in cont
We were kindly provided by Z. al-Doori (Dept. of Molecular Biology, University of Edinburgh) with a hybrid plasmid produced by in vitro techniques, pZD23 (A1- Doori et al. 1982). This plasmid is derived from pZD100, which consists of a 2drpoBC18 (=2drpoB3) prophage inserted into RP42att, by a deleti