Three temperature-sensitive mutant strains for RNA polymerase beta or beta' subunits (carrying mutations tsx, A2R7 and R120) were used in order to investigate the dependence of the induced lac expression on stimulation by cyclic AMP after the shift to non-permissive temperature. High temperature low
Messenger RNA Stability and Polarity in the lac Operon of Escherichia coli
β Scribed by CARTER, TIMOTHY; NEWTON, AUSTIN
- Book ID
- 109668761
- Publisher
- Nature Publishing Group
- Year
- 1969
- Tongue
- English
- Weight
- 414 KB
- Volume
- 223
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/223707a0
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The transcriptional and translational events occurring during the induction of the lac operon, were separated by blocking the translational step, either by aminoacid starvation or by addition of chloramphenicol. It was found that the carbon source used during the subsequent translation, affected the
Escherichia coli, cultured on minimal medium and deprived of its required amino-acids, was induced for lac genes transcription. After inducer removal and restoration of growth, beta-galactosidase synthesis was measured. Two different kinetics of enzyme synthesis were observed depending on the starva