New assay to detect shedding antigen with use of anti-idiotypic monoclonal antibodies

✍ Scribed by Masayuki Tsujisaki; Kohzoh Imai; Shigeru Tokuchi; Jiroh Masuya; Tadao Ishida; Masaaki Adachi; Yuji Hinoda; Akira Yachi; Yoshiaki Hanzawa

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 627 KB
• Volume: 5
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860050104

✦ Synopsis

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Anti-idiotypic (Anti-Id) monoclonal antibodies (MoAbs) against anti-carcinoembryonic antigen (CEA) monoclonal antibody 563 (IgG1) were prepared and characterized. Five anti-Id MoAbs recognized the private idiotype of MoAb 563. ldiotype mapping suggested that MoAb 563 had at least three distinct idiotopes at its antigen combining site.

Utilizing the inhibition of idiotype-anti-idiotype interaction with use of anti-id MoAb T4-305 or T4-212, an immunoassay to detect shedding CEA was established. The results of this inhibition assay correlated with those of double determinant immunoassay and inhibition percentages linearly increased in a CEA concentration-dependent manner.

Since it requires only one epitope of a given antigen molecule, this assay could be widely used for detection of shedding antigens, including tumor-associated antigens.