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New assay for tyrosine aminotransferase with the amino acid analyzer

✍ Scribed by E.A. Lane; C. Mavrides

Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
259 KB
Volume
44
Category
Article
ISSN
0003-2697

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✦ Synopsis

Most assay methods for tyrosine aminotransferase (n-tyrosine:2-oxoglutarate aminotransferase, EC 2.6.1.5) measure the formation of p-hydroxyphenylpyruvic acid (pHPP). The estimation of pHPP is based on the formation of colored products (l-3)) the enol borate-tautomerase reaction (4), the formation of p-hydroxybenzaldehyde from pHPP (5), or the counting of radioactivity derived from the isotopic substrate n-tyrosine (6-8). A recent method is based on the chromatographic separation on cation-exchange paper of 14C-n-glutamic acid formed from W-2-0x0glutaric acid (9).

In recent studies in our laboratory the assay of tyrosine aminotransferase by the estimation of the glutamic acid formed was desirable. For this reason we developed a reliable sensitive method in which n-glutamic acid is separated and estimated with the amino acid analyzer.

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