New method for quantitative determinatio
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Jean-Marc Navenot; Taha Merghoub; Rolande Ducrocq; Jean-Yves Muller; Rajagopal K
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Article
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1998
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John Wiley and Sons
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English
โ 78 KB
๐ 2 views
A new method to quantify red blood cells (RBCs) containing fetal hemoglobin (HbF), or F cells, by flow cytometry was developed. The use of formaldehyde as fixative agent and sodium dodecyl sulfate (SDS) to permeabilize fixed RBCs resulted in a low staining background, a negligible Hb leakage, and mi