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New applications of flow cytometry

โœ Scribed by Ger van den Engh


Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
718 KB
Volume
4
Category
Article
ISSN
0958-1669

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A new method to quantify red blood cells (RBCs) containing fetal hemoglobin (HbF), or F cells, by flow cytometry was developed. The use of formaldehyde as fixative agent and sodium dodecyl sulfate (SDS) to permeabilize fixed RBCs resulted in a low staining background, a negligible Hb leakage, and mi