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Flow cytometry: New guidelines to support its clinical application

โœ Scribed by Melissa D'Archangelo


Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
71 KB
Volume
72B
Category
Article
ISSN
1552-4949

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A new method to quantify red blood cells (RBCs) containing fetal hemoglobin (HbF), or F cells, by flow cytometry was developed. The use of formaldehyde as fixative agent and sodium dodecyl sulfate (SDS) to permeabilize fixed RBCs resulted in a low staining background, a negligible Hb leakage, and mi