An industrial application of multiparameter flow cytometry: Assessment of cell physiological state and its application to the study of microbial fermentations
✍ Scribed by Christopher J. Hewitt; Gerhard Nebe-Von-Caron
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 337 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0196-4763
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Multiparameter flow cytometric techniques developed in our laboratories have been used for the "atline" study of fed-batch bacterial fermentations. These fermentations were done at two scales, production (20 m 3 ) and bench (5 × 10 -3 m 3 ). In addition, at the bench scale, experiments were undertak
## Abstract Co‐immunoprecipitation is the classical approach for investigating protein–protein interactions. Analysis is generally conducted using the Western blot approach. We set out to investigate whether flow cytometry was a feasible alternative to Western blotting. Using the TCR‐CD3 complex as