New and sensitive reaction for automatic determination of inorganic phosphate and its application to serum
β Scribed by H. Van Belle
- Publisher
- Elsevier Science
- Year
- 1970
- Tongue
- English
- Weight
- 546 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A new and convenient method for the determination of P, was developed. Phosphomolybdate is measured calorimetrically, without reduction to molybdenum blue, by dissolving the whole assay mixture in acetone, where phosphomolybdate is bright yellow. The hydrolysis of acidlabile phosphates (e.g., creati
We present here an improvement in the classical molybdate method for inorganic phosphate determination. This method has high sensitivity, with 1 nmol Of Pi giving an absorbance change of 0.060 &a unit. It is highly reproducible and the color remains stable for at least 3.5 h. In addition the use of
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