Neutral amino acid transport during the cell cycle of cultured human lymphocytes

✍ Scribed by Mark C. Glassy; Clement E. Furlong

• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: English
• Weight: 513 KB
• Volume: 107
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041070109

✦ Synopsis

Abstract

Neutral amino acid (isoleucine, leucine, and valine) transport was monitored in synchronous populations of WIL‐2 cells, a diploid human lymphoblastoid B cell line. Cells were synchronized by either the double thymidine block technique or by density‐dependent arrest in G~1~. Cells synchronized by these methods showed up to a 66% decrease in amino acid transport upon progressing from the early S phase through the G~2~ and M phases with a concomitant increase in transport during the G~1~ phase. The lowest activities of amino acid transport in synchronized cells were observed during the G~2~ and M phases.