Neurotransmitter Transporters

✍ Scribed by Susan G. Amara (Eds.)

• Publisher: Academic Press
• Year: 1998
• Tongue: English
• Leaves: 824
• Series: Methods in Enzymology 296
• Edition: 1
• Category: Library

✦ Synopsis

General Description of the Series:
Neurotransmitter Transporters focuses on biochemical, electrophysiological, pharmacological, molecular, and cell biological approaches used to study neurotransmitter transport systems. The articles provide detailed descriptions of procedures that should enable the reader to understand how they are accomplished and to repeat or adapt them for their own experimental needs. This book is the first to focus on methods that have been the basis for the rapid development of this area.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Key Features
The transport of CNS neurotransmitter transporters
Electrophysiological, biochemical, molecular, cellular biological, pharmacological, neurochemical, and structural approaches
* Both plasma and vesicular carriers

✦ Table of Contents

Content:
Contributors to volume 296
Pages ix-xiii

Preface
Page xv
Susan G. Amara

Volumes in series
Pages xvii-xxxiv

[1] Purification, hydrodynamic properties, and glycosylation analysis of glycine transporters Original Research Article
Pages 3-17
Carmen AragГіn, Beatriz LГіpez-Corcuera

[2] Expression cloning using Xenopus laevis oocytes Original Research Article
Pages 17-52
Michael F Romero, Yoshikatsu Kanai, Hiromi Gunshin, Matthias A Hediger

[3] Cloning of genes or cDNAs encoding neurotransmitter transporters and their localization by immunocytochemistry Original Research Article
Pages 52-64
Nathan Nelson, Qing-Rong Liu

[4] Purification of vesicular monoamine transporters: From classical techniques to histidine tags Original Research Article
Pages 64-72
Rodrigo Yelin, Shimon Schuldiner

[5] Noncovalent and covalent labeling of vesicular monoamine transporter with tetrabenazine and ketanserin derivatives: Purification of photolabeled protein Original Research Article
Pages 73-83
Jean-Pierre Henry, Corinne Sagné, Marie-Françoise Isambert, Bruno Gasnier

[6] Functional identification of vesicular monoamine and acetylcholine transporters Original Research Article
Pages 84-99
Helene Varoqui, Jeffrey D. Erickson

[7] Photoaffinity labeling of vesicular acetylcholine transporter from electric organ of Torpedo Original Research Article
Pages 99-116
Stanley M. Parsons, Gary A. Rogers, Lawrence M. Gracz

[8] Bioenergetic characterization of Оі-aminobutyric acid transporter of synaptic vesicles Original Research Article
Pages 116-124
Johannes W. Hell, Reinhard Jahn

[9] Solubilization and reconstitution of synaptic vesicle glutamate transport system Original Research Article
Pages 125-144
Scott M. Lewis, Tetsufumi Ueda

[10] Analysis of neurotransmitter transport into secretory vesicles Original Research Article
Pages 145-162
J.Patrick Finn III, Andrew Merickel, Robert H. Edwards

[11] Inhibitors of Оі-aminobutyric acid transport as experimental tools and therapeutic agents Original Research Article
Pages 165-175
Povl Krogsgaard-Larsen, Bente F. FrГёlund, Erik Falch

[12] Design and synthesis of conformationally constrained inhibitors of high-affinity, sodium-dependent glutamate transporters Original Research Article
Pages 175-189
A. Richard Chamberlin, Hans P. Koch, Richard J. Bridges

[13] Examination of glutamate transporter heterogeneity using synaptosomal preparations Original Research Article
Pages 189-202
Michael B. Robinson

[14] Specificity and ion dependence of binding of GBR analogs Original Research Article
Pages 203-219
Amadou T. Corera, Jean-Claude Do RГ©go, Jean-Jacques Bonnet

[15] Cocaine and GBR photoaffinity labels as probes of dopamine transporter structure Original Research Article
Pages 219-230
Roxanne A. Vaughan

[16] Ion-coupled neurotransmitter transport: Thermodynamic vs. kinetic determinations of stoichiometry Original Research Article
Pages 233-247
Gary Rudnick

[17] Inhibition of [³H]dopamine translocation and [³H]cocaine analog binding: A potential screening device for cocaine antagonists Original Research Article
Pages 248-259
Maarten E.A. Reith, Cen Xu, F. Ivy Carroll, Nian-Hang Chen

[18] Transport and drug binding kinetics in membrane vesicle preparation Original Research Article
Pages 259-278
Heinz Bönisch

[19] Use of human placenta in studies of monoamine transporters Original Research Article
Pages 278-290
Vadivel Ganapathy, Puttur D. Prasad, Frederick H. Leibach

[20] Analysis of transporter topology using deletion and epitope tagging Original Research Article
Pages 293-307
Janet A. Clark

[21] Selective labeling of neurotransmitter transporters at the cell surface Original Research Article
Pages 307-318
Gwynn M. Daniels, Susan G. Amara

[22] Transmembrane topology mapping using biotin-containing sulfhydryl reagents Original Research Article
Pages 318-331
Rebecca P. Seal, Barbara H. Leighton, Susan G. Amara

[23] Probing structure of neurotransmitter transporters by substituted-cysteine accessibility method Original Research Article
Pages 331-346
Jonathan A. Javitch

[24] Biosynthesis, N-glycosylation, and surface trafficking of biogenic amine transporter proteins Original Research Article
Pages 347-370
Sammanda Ramamoorthy, Haley E. Melikian, Yan Qian, Randy D. Blakely

[25] Expression of neurotransmitter transport systems in polarized cells Original Research Article
Pages 370-388
Jinhi Ahn, Grazia Pietrini, Theodore R. Muth, Michael J. Caplan

[26] Localization of transporters using transporter-specific antibodies Original Research Article
Pages 388-407
N.C. Danbolt, K.P. Lehre, Y. Dehnes, F.A. Chaudhry, L.M. Levy

[27] Generation of transporter-specific antibodies Original Research Article
Pages 407-422
Gary W. Miller, Michelle L. Gilmor, Allan I. Levey

[28] Homologies and family relationships among Na⁺/Cl^в€’ neurotransmitter transporters Original Research Article
Pages 425-436
Holger Lill, Nathan Nelson

[29] Vaccinia virus-T7 RNA polymerase expression system for neurotransmitter transporters Original Research Article
Pages 436-443
Sue L. Povlock, Susan G. Amara

[30] Baculovirus-mediated expression of neurotransmitter transporters Original Research Article
Pages 443-455
Christopher G. Tate

[31] Dopamine transporter mutants, small molecules, and approaches to cocaine antagonist/dopamine transporter disinhibitor development Original Research Article
Pages 456-465
George Uhl, Zhicheng Lin, Thomas Mitzger, Dalit E. Dar

[32] In vivo generation of chimeras Original Research Article
Pages 466-475
Kari Johnson Buck, Susan G. Amara

[33] Structural determinants of neurotransmitter transport using cross-species chimeras: studies on serotonin transporter Original Research Article
Pages 475-498
Eric L. Barker, Randy D. Blakely

[34] Molecular cloning of neurotransmitter transporter genes: Beyond coding region of cDNA Original Research Article
Pages 498-514
David J. Vandenbergh

[35] Use of antisense oligodeoxynucleotides to inhibit expression of glutamate transporter subtypes Original Research Article
Pages 514-529
Lynn A. Bristol, Jeffrey D. Rothstein

[36] Using Caenorhabditis elegans to study vesicular transport Original Research Article
Pages 529-532,IN1,533-547
James B. Rand, Janet S. Duerr, Dennis L. Frisby

[37] Measurement of transient currents from neurotransmitter transporters expressed in Xenopus oocytes Original Research Article
Pages 551-566
Sela Mager, Yongwei Cao, Henry A. Lester

[38] Fluorescence techniques for studying cloned channels and transporters expressed in Xenopus oocytes Original Research Article
Pages 566-578
Albert Cha, Noa Zerangue, Michael Kavanaugh, Francisco Bezanilla

[39] Fluctuation analysis of norepinephrine and serotonin transporter currents Original Research Article
Pages 578-593
Louis J. DeFelice, Aurelio Galli

[40] Serotonin transport in cultured leech neurons Original Research Article
Pages 593-607
Dieter Bruns

[41] Glutamate uptake in Purkinje cells in rat cerebellar slices Original Research Article
Pages 608-617
Michiko Takahashi, Monique Sarantis, David Attwell

[42] Patch-clamp, ion-sensing, and glutamate-sensing techniques to study glutamate transport in isolated retinal glial cells Original Research Article
Pages 617-632
Brian Billups, Marek Szatkowski, David Rossi, David Attwell

[43] Measurement of glial transport currents in microcultures: Application to excitatory neurotransmission Original Research Article
Pages 632-645
Steven Mennerick, Charles F. Zorumski

[44] Voltammetric studies on kinetics of uptake and efflux at catecholamine transporters Original Research Article
Pages 649-660
Krisstina Danek, Jay B. Justice Jr.

[45] Resolution of biogenic amine transporter kinetics by rotating disk electrode voltammetry: Methodology and mechanistic interpretations Original Research Article
Pages 660-675
Cynthia Earles, Hollie Wayment, Mitchell Green, James O. Schenk

[46] Electrochemical detection of reverse transport from Planorbis giant dopamine neuron Original Research Article
Pages 675-689
Brian B. Anderson, Andrew G. Ewing, David Sulzer

[47] Measuring uptake rates in intact tissue Original Research Article
Pages 689-707
Melissa A. Bunin, R. Mark Wightman

[48] High-speed chronoamperometric electrochemical measurements of dopamine clearance Original Research Article
Pages 708-719
Nancy R. Zahniser, Shelly D. Dickinson, Greg A. Gerhardt

[49] In Vivo microdialysis for measurement of extracellular monoamine levels following inhibition of monoamine transporters Original Research Article
Pages 719-730
Nian-Hang Chen, Maarten E.A. Reith

Author index
Pages 731-762

Subject index
Pages 763-784