Neural Stem Cells: Methods and Protocols

✍ Scribed by Leslie P. Weiner

• Publisher: Humana Press
• Year: 2008
• Tongue: English
• Leaves: 405
• Series: Methods in Molecular Biology
• Edition: 2nd
• Category: Library

✦ Synopsis

Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger.  Neural Stem Cells, 2nd Edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions.  The use of these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models.  In addition to the topics covered in the first edition, this new volume provides recently developed alternative methods for both human and rodent NSCs, along with chapters on karyotyping, migration, apoptosis, and NSC markers. Following the Methods in Molecular Biology™ series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and current, Neural Stem Cells, 2nd Edition is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases.

✦ Table of Contents

Cover......Page 1
Frontmatter......Page 2
I - Introduction......Page 16
1 Definitions and Criteria for Stem Cells......Page 17
2 Neural Stem Cells and the Future Treatment of Neurological Diseases: Raising the Standard......Page 23
II - Isolation and Culture of NSCs......Page 31
3 Neural Differentiation of Human Embryonic Stem Cells......Page 32
4 Generating Neurons from Stem Cells......Page 44
5 Isolation of Neural Stem and Precursor Cells from Rodent Tissue......Page 52
6 Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation and Characterization......Page 67
7 Adult Neural Stem Cells......Page 79
8 Olfactory Ensheathing Cells: Isolation and Culture from the Neonatal Olfactory Bulb......Page 97
9 Culturing Olfactory Ensheathing Cells from the Mouse Olfactory Epithelium......Page 107
10 Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property......Page 115
11 Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood......Page 134
12 Production of Neurospheres from CNS Tissue......Page 146
13 Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells......Page 162
III - In Vitro Studies: A. Cellular Techniques......Page 181
14 Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures......Page 182
15 Telomere Neurobiology......Page 194
16 Chromosomal Mosaicism in Neural Stem Cells......Page 206
17 In Vitro Assays for Neural Stem Cell Differentiation: Induction of Dopaminergic Phenotype......Page 214
18 In Vitro Migration Assays of Neural Stem Cells......Page 222
19 Measuring Apoptosis in Neural Stem Cells......Page 235
20 Markers of Adult Neural Stem Cells......Page 250
III - In Vitro Studies: B. Molecular Techniques......Page 276
21 Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR......Page 277
22 Microfluidic Devices for High-Throughput Gene Expression Profiling of Single hESC-Derived Neural Stem Cells......Page 298
23 Electroporation Strategies for Genetic Manipulation and Cell Labeling......Page 309
24 Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene Therapy Approaches......Page 322
IV - Studies for Transplantation......Page 336
25 Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo......Page 337
26 Immunocytochemical Analysis of Neuronal Differentiation......Page 346
27 Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold......Page 354
28 Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS......Page 361
29 Optimizing Stem Cell Grafting into the CNS......Page 375
30 Routes of Stem Cell Administration in the Adult Rodent......Page 383
Index......Page 402

✦ Subjects

Психологические дисциплины;Нейропсихология;