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Neural Stem Cells: Methods and Protocols

✍ Scribed by Marcel M. Daadi

Publisher
Springer New York;Humana Press
Year
2019
Tongue
English
Leaves
242
Series
Methods in Molecular Biology 1919
Edition
1st ed.
Category
Library
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✦ Synopsis

This volume details various technological advances to isolate, perpetuate, and characterize neural stem cells from various sources and formulates them for the delivery into animal models of neurological disorders. Chapters guide the reader through methods and protocols on definition and isolation of neural stem cells, characterization and differentiation, standards for defining cell populations, and In vivo approaches in animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Neural Stem Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field.

✦ Table of Contents

Front Matter ....Pages i-xiv
Generation of Neural Stem Cells from Induced Pluripotent Stem Cells (Marcel M. Daadi)....Pages 1-7
Making NSC and Neurons from Patient-Derived Tissue Samples (Odity Mukherjee, Shubhra Acharya, Mahendra Rao)....Pages 9-24
Generation of Definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury (Mohamad Khazaei, Christopher S. Ahuja, Christopher E. Rodgers, Priscilla Chan, Michael G. Fehlings)....Pages 25-41
Derivation of Neural Stem Cells from Human Parthenogenetic Stem Cells (Rodolfo Gonzalez, Ibon Garitaonandia, Andrey Semechkin, Russell Kern)....Pages 43-57
Chemically Defined Neural Conversion of Human Pluripotent Stem Cells (Yu Chen, Carlos A. Tristan, Sunil K. Mallanna, Pinar Ormanoglu, Steven Titus, Anton Simeonov et al.)....Pages 59-72
In Vitro Functional Characterization of Human Neurons and Astrocytes Using Calcium Imaging and Electrophysiology (Marita GrΓΈnning Hansen, Daniel Tornero, Isaac Canals, Henrik Ahlenius, Zaal Kokaia)....Pages 73-88
Differentiation of Neural Stem Cells Derived from Induced Pluripotent Stem Cells into Dopaminergic Neurons (Marcel M. Daadi)....Pages 89-96
Midbrain Dopaminergic Neurons Differentiated from Human-Induced Pluripotent Stem Cells (Fabiano Araújo Tofoli, Ana Teresa Silva Semeano, Ágatha Oliveira-Giacomelli, Maria Carolina Bittencourt Gonçalves, Merari F. R. Ferrari, Lygia Veiga Pereira et al.)....Pages 97-118
Generating Neural Stem Cells from iPSCs with Dopaminergic Neurons Reporter Gene (Hyenjong Hong, Marcel M. Daadi)....Pages 119-128
Single-Cell Library Preparation of iPSC-Derived Neural Stem Cells (Jeffrey Kim, Marcel M. Daadi)....Pages 129-143
Bioinformatics Analysis of Single-Cell RNA-Seq Raw Data from iPSC-Derived Neural Stem Cells (Jeffrey Kim, Marcel M. Daadi)....Pages 145-159
Assay for Assessing Mitochondrial Function in iPSC-Derived Neural Stem Cells and Dopaminergic Neurons (Gourav Roy-Choudhury, Marcel M. Daadi)....Pages 161-173
Reference Transcriptome for Deriving Marmoset Induced Pluripotent Stem Cells (Guang Yang, Hyenjong Hong, April Torres, Kristen E. Malloy, Gourav Roy-Choudhury, Jeffrey Kim et al.)....Pages 175-186
Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach (Steven L. Farnsworth, Zhifang Qiu, Anuja Mishra, Peter J. Hornsby)....Pages 187-197
Isolation and Differentiation of Self-Renewable Neural Stem Cells from Marmoset-Induced Pluripotent Stem Cells (Hyenjong Hong, Gourav Roy-Choudhury, Jeffrey Kim, Marcel M. Daadi)....Pages 199-204
Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation (Cang Chen, Michael J. Guderyon, Guo Ge, Robert A. Clark, Senlin Li)....Pages 205-213
Central and Peripheral Secondary Cell Death Processes after Transient Global Ischemia in Nonhuman Primate Cerebellum and Heart (Jea-Young Lee, Roger Lin, Hung Nguyen, Eleonora Russo, M. Grant Liska, Trenton Lippert et al.)....Pages 215-225
Method for Stimulation of Hippocampal Neurogenesis by Transient Microneedle Insertion (Shijie Song, Xiaoyung Kong, Juan Sanchez-Ramos)....Pages 227-235
Back Matter ....Pages 237-239

✦ Subjects

Biomedicine; Neurosciences; Stem Cells; Animal Models

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