✦ LIBER ✦

Nerve growth factor-stimulated calcium uptake into PC12 cells: Uniqueness of the channel and evidence for phosphorylation

✍ Scribed by B. Nikodijevic; G. Guroff

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 886 KB
Volume: 31
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490310402

No coin nor oath required. For personal study only.

✦ Synopsis

Nerve growth factor stimulates the uptake of radioactive calcium into PC12 cells. This stimulation is inhibited by low concentrations of dideoxyforskolin or staurosporine, and by high concentrations of nifedipine or cadmium. On the other hand, neither dideoxyforskolin nor staurosporine inhibited the stimulation of calcium uptake caused by BK-8644 or adenosine triphosphate (ATP). Nickel inhibited only the effect of ATP on calcium uptake, and actually stimulated the effects of either BK-8644 or nerve growth factor.

Down-regulation of L-calcium channels by BK-8644 blocked the subsequent stimulation of calcium uptake by this agent, but not the stimulation by nerve growth factor. Conversely, pre-treatment of the cells with nerve growth factor inhibited the subsequent stimulation of calcium uptake by nerve growth factor, but not the stimulation by BK-8644. The effects of BK-8644 and nerve growth factor on calcium uptake were additive, as were the effects of nerve growth factor and ATP.

Phosphatase 2A inhibited the effect of nerve growth factor on calcium uptake, but did not influence the action of BK-8644. On the other hand, calcineurin inhibited the effect of BK-8644 on calcium uptake, but potentiated the action of nerve growth factor. Calmidazolium or fluphenazine also inhibited the effect of nerve growth factor on calcium uptake, but okadaic acid stimulated it.

A comparison of the effects of these inhibitors on the actions of various calcium channel agonists shows that the channels on which the action of nerve growth factor is exerted are different than either the L-type calcium channels or the ATP-activated calcium channels. These data also indicate that the action of nerve growth factor is mediated by phosphorylation of some component of the nerve growth factor-sensitive calcium channel.

📜 SIMILAR VOLUMES

Involvement of protein kinase C in nerve

Involvement of protein kinase C in nerve growth factor- and K-252a-stimulated calcium uptake into PC12 cells

✍ Geneva Dickens; Miriam Lavarreda; Wen-Hua Zheng; Gordon Guroff 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 75 KB 👁 2 views

Both nerve growth factor (NGF) and K-252a stimulate the uptake of calcium into PC12 cells. Stimulation by either is prevented by pretreatment of the cells with the tumor promoter phorbol 12-myristate 13-acetate (PMA), suggesting an involvement of protein kinase C in the stimulation. The effect of PM

Intracellular calcium levels regulate th

Intracellular calcium levels regulate the actions of nerve growth factor on calcium uptake in PC12 cells

✍ A. Kozak; B. Nikodijevic; E. Yavin; G. Guroff 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 637 KB

The uptake of divalent cations and the intracellular concentration of calcium in PC12 cells were studied by flow cytometric analysis using the calcium-sensitive dye, Fluo-3, under a variety of conditions. In particular the actions of nerve growth factor were analyzed. The data show that nerve growth

Threonine 308 phosphorylated form of akt

Threonine 308 phosphorylated form of akt translocates to the nucleus of PC12 cells under nerve growth factor stimulation and associates with the nuclear matrix protein nucleolin

✍ Paola Borgatti; Alberto M. Martelli; Giovanna Tabellini; Alfonso Bellacosa; Silv 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 321 KB

## Abstract We have examined the issue of whether or not in PC12 cells it may be observed a nerve growth factor (NGF) nuclear translocation of an active (phosphorylated) Akt. Western blot analysis with antibodies to either total or phosphorylated Akt showed a maximal nuclear translocation after 15