Involvement of protein kinase C in nerve growth factor- and K-252a-stimulated calcium uptake into PC12 cells

✍ Scribed by Geneva Dickens; Miriam Lavarreda; Wen-Hua Zheng; Gordon Guroff

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 75 KB
• Volume: 47
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/(sici)1097-4547(19970201)47:3<271::aid-jnr5>3.0.co;2-f

✦ Synopsis

Both nerve growth factor (NGF) and K-252a stimulate the uptake of calcium into PC12 cells. Stimulation by either is prevented by pretreatment of the cells with the tumor promoter phorbol 12-myristate 13-acetate (PMA), suggesting an involvement of protein kinase C in the stimulation. The effect of PMA is specific in that the calcium uptake stimulated by either the L-type channel agonist BAY K 8644 or by ATP is not altered in PMA-pretreated cells. An involvement of kinase C is also suggested by the inhibition of NGF-or K-252astimulated calcium uptake by the kinase C inhibitors staurosporine and calphostin C. Inhibition by the isoform-specific agents GO 6976 and thymeleatoxin implicates one of the classic calcium-sensitive isoforms of kinase C. The close similarity in the profiles of inhibition of NGF-stimulated and K-252a-stimulated calcium uptake by the various effectors suggests that NGF and K-252a act on calcium uptake through some of the same signaling elements.