𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Neoplastic transformation of a human kidney epithelial cell line transfected with V-HA-ras oncogene

✍ Scribed by Aage Haugen; David Ryberg; Inger-Lise Hansteen; Paul Amstad

Publisher
John Wiley and Sons
Year
1990
Tongue
French
Weight
724 KB
Volume
45
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

We have recently shown that normal human kidney epithelial (NHKE) cells were immortalized by treatment with Ni(II) alone. In the present study the immortalized human kidney cell line (IHKE) was transfected with a plasmid construct containing the v‐Ha‐ras oncogene (pZip__ras__). After transfection, the cell lines formed tumors in athymic nude mice, whereas the ZipNeoSV(X)‐transfected IHKE control cultures formed no tumors. Tumor cell lines (THKE) were established from the tumors in nude mice. These cells appear to be of human epithelial origin and express high levels of Ha‐ras transcript. Karyotypic analysis was performed. The cell lines were tritetra‐ or pentaploid. A consistent finding in the IHKE, IHKZE and THKE cells was increased numbers of chromosomes 17 and 7p+. Some marker chromosomes were identical in the IHKE and THKE cell lines, underlining their common origin and their possible importance in the carcinogenic process. This shows that the combined action of a chemical carcinogen [i.e., Ni(II)] and v‐Ha‐ras oncogene resulted in fully transformed human kidney epithelial cells, consistent with a stepwise progression of human epithelial cell transformation.

📜 SIMILAR VOLUMES

Malignant transformation of human bladde
Malignant transformation of human bladder epithelial cells by DNA transfection with the v-raf oncogene
✍ Jan Skouv; Svend Ottesen; George Mark; Herman Autrup 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 448 KB

Transfection of the v-raf oncogene into immortalized, nontumorigenic human bladder epithelial cells resulted in the isolation of two tumorigenic transformants. Both were identified as human and of the same origin as the parent cell line by human leukocyte antigen typing and Southern blot analysis. B

Induction of multidrug resistance (MDR)
Induction of multidrug resistance (MDR) by transfection of MCF-10A cell line with c-Ha-ras and c-erbB-2 oncogenes
✍ Antonietta R. M. Sabbatini; Fulvio Basolo; Paola Valentini; Letizia Mattii; Simo 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 French ⚖ 595 KB

To investigate the relationship between oncogene activation and appearance of rnultidrug resistance (MDR) we transfected the human breast epithelial cell line MCF-I OA, negative for the expression of the P-glycoprotein, with c-Ha-ras and/or c-erb6-2 oncogenes. The appearance of the MDR phenotype was

Allelic imbalance at 11p15.5–15.4 correl
Allelic imbalance at 11p15.5–15.4 correlated with c-Ha-ras mutation during radiation-induced neoplastic transformation of human breast epithelial cells
✍ Debasish Roy; Gloria Calaf; Tom K. Hei 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 French ⚖ 305 KB

## Abstract Breast cancer is the most frequent malignancy in women throughout much of the developed world and is associated with a multistage process involving a number of genetic mutations and their corresponding cellular phenotypic alterations. It has already been shown that neoplastic transforma

Analysis of v-Ha-ras and v-fos oncogene
Analysis of v-Ha-ras and v-fos oncogene transduction into a mouse epidermal cell line with “initiated” phenotype in culture but normal skin phenotype in vivo
✍ Masato Ueda; Hideki Kawamura; Christian Sutter; Adam Glick; Stuart H. Yuspa; Jam 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 679 KB

## Abstract Cell line SCR722 was derived from adult SENCAR mouse epidermal cells initiated in culture by treatment with the carcinogen __N__‐methyl‐__N__′‐nitro‐N‐nitrosoguanidine and selection for foci proliferating in medium with calcium levels that induce terminal differentiation in normal cells