Induction of multidrug resistance (MDR) by transfection of MCF-10A cell line with c-Ha-ras and c-erbB-2 oncogenes
✍ Scribed by Antonietta R. M. Sabbatini; Fulvio Basolo; Paola Valentini; Letizia Mattii; Simonetta Calvo; Lisa Fiore; Fortunato Ciardiello; Mario Petrini
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- French
- Weight
- 595 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
To investigate the relationship between oncogene activation and appearance of rnultidrug resistance (MDR) we transfected the human breast epithelial cell line MCF-I OA, negative for the expression of the P-glycoprotein, with c-Ha-ras and/or c-erb6-2 oncogenes. The appearance of the MDR phenotype was then studied by evaluating mdr-I mRNA expression, the presence of P-glycoprotein on the cell membrane and the onset of doxorubicin resistance, together with the effect of the reversing agent verapamil. We found that only MCF-I OA transfected with both c-Ha-ras and c-erbB-2 oncogenes acquired the MDR phenotype. o I994 Wiley-Liss, Inc.