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Negative silver staining in A-T and satellite DNA-rich regions of human chromosomes

✍ Scribed by William Mike Howell; Thomas E. Denton

Publisher: Springer
Year: 1976
Tongue: English
Weight: 662 KB
Volume: 57
Category: Article
ISSN: 0009-5915
DOI: 10.1007/bf00292914

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✦ Synopsis

Human metaphase chromosomes were stained with silver following a pretreatment with a heated alkaline solution. The most conspicuous feature of the stained metaphases was the omission of silver staining in the secondary constrictions of chromosomes 1,9 and 16, and on the distal Yq. Our evidence indicates that the negative silver binding is due to the preferential removal or alteration of non-histone proteins associated with these regions. The cytochemical significance of these findings is discussed.

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