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Nearest-neighbor and next-nearest-neighbor effects in the proton NMR spectra of the oligoribonucleotides ApXpG, CpXpG, CpApXpUpG, ApGpXpC, and ApGpXpCpU

✍ Scribed by Russell A. Bell; Jeremy R. Everett; Donald W. Hughes; Dirk Alkema; Paul Hader; Thomas Neilson; Paul J. Romaniuk

Publisher
Wiley (John Wiley & Sons)
Year
1981
Tongue
English
Weight
679 KB
Volume
20
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

The proton nmr spectra of the oligoribonucleotides in the series Cp__X__pG, Ap__X__pG, CpAp__X__pUpG, and ApGp__X__pC (X = A, G, C, and U), together with the reference compounds CpG, ApG, CpApUpG, and ApGpC, have been measured. A complete analysis of all the nonexchangeable base protons and the ribose H‐1′ protons was made. The insertion of a nucleotide X into a oligoribonucleotide led to shift changes at both nearest‐neighbor and next‐nearest‐neighbor positions, which were rationalized in terms of the shielding abilities of the various bases. The derived shielding trends in the ApGp__X__pC series of compounds were successfully used to predict the chemical shifts of resonances in the related ApGp__X__pCpU series.

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