Natural plant enzyme inhibitors. α-amylase inhibitors in millets
✍ Scribed by Gudiseva Chandrasekher; D. Suryaprasad Raju; Thillaisthanam N. Pattabiraman
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 431 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0022-5142
No coin nor oath required. For personal study only.
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## Abstract Two α‐amylase inhibitors I‐1 and I‐2, were purified to homogeneity from __Colocasia antiquorum__ tubers by extraction with 0.02m phosphate buffer pH 7.6, treatment with diethyl amino ethyl‐cellulose, ammonium sulphate fractionation, chromatography on Sephadex G‐50 and chromatography on
## Abstract A high‐molecular‐weight α‐amylase inhibitor has been isolated from mature tubers of __Dioscorea alata__ by extraction with 0.02M acetate buffer (pH 5.0), negative absorption on CM‐cellulose and ultracentrifuging. The inhibitor was fairly heat stable and was active against human salivary