Nanofiltration membranes based on poly(vinyl alcohol) (PVA) and ionic polymers, such as sodium alginate (SA) and chitosan, were prepared by casting the respective polymer solutions. The membranes prepared from PVA or PVA-ionic polymer blend were crosslinked in a isopropanol solution using glutaraldehyde as a crosslinking agent. The membranes were characterized with Fourier transform infrared spectroscopy and X-ray diffractometry and swelling test. The membranes crosslinked through the acetal linkage formation between the OOH groups of PVA and the ionomer and glutaraldehyde appeared to be semicrystalline. To study the permeation properties, the membranes were tested with various feed solutions [sodium sulfate, sodium chloride, poly(ethylene glycol) with 600 g/mol of molecular weight (PEG 600), and isopropyl alcohol]. For example, the permeance and the solute rejection of the 1000 ppm sodium sulfate at 600 psi of upstream pressure through the PVA membrane were 0.55 m 3 /m 2 day and over 99%, respectively. The effects of the ionomers on the permeation properties of the PVA membranes were studied using the PVA-SA and PVA-chitosan blend membranes. The addition of small amount of ionic polymers (5 wt %) made the PVA membranes more effective for the organic solute rejection without decrease in their fluxes. The rejection ratios of the PEG 600 and isopropanol were increased substantially.