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Nanocapsule-based probe for evaluating the orientation of antibodies immobilized on a solid phase

✍ Scribed by Iijima, Masumi; Yoshimoto, Nobuo; Niimi, Tomoaki; Maturana, Andrés Daniel; Kuroda, Shun'ichi

Book ID
120318332
Publisher
Royal Society of Chemistry
Year
2013
Tongue
English
Weight
653 KB
Volume
138
Category
Article
ISSN
0003-2654

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✍ Arti Ahluwalia; Danilo De Rossi; Giuseppe Giusto; Oren Chen; Vladislav Papper; G 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 251 KB

A fluorescent-photochrome method of quantifying the orientation and surface density of solid phase antibodies is described. The method is based on measurements of quenching and rates of cis-trans photoisomerization and photodestruction of a stilbene-labeled hapten by a quencher in solution. These ex