N-terminal sequence of phage lambda repressor
โ Scribed by Beyreuther, Konrad ;Gronenborn, Bruno
- Book ID
- 104729190
- Publisher
- Springer
- Year
- 1976
- Tongue
- English
- Weight
- 264 KB
- Volume
- 147
- Category
- Article
- ISSN
- 0026-8925
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โฆ Synopsis
Lambda repressor was purified from an E. coli strain which produces 150 times more lambda repressor than a single lysogen. The sequence of the fifty N-terminal residues was determined by automated Edman degradation. It contains 43% of all arginine and lysine residues of the chain and constitutes according to the genetic data of Oppenheim et al. (1975) a substantial part of the operator-DNA-binding site of the repressor.
๐ SIMILAR VOLUMES
The gene coding for bacteriophage Lambda repressor (cI gene) has been fused to the lac operon of Escherichia coli. In some of the fusions Lambda repressor synthesis can be controlled by the lac operator and promoter. Upon induction of the lac operon the amount of Lambda repressor is increased by a f