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N-terminal and C-terminal modifications affect folding, release from the ribosomes and stability of in vitro synthesized proteins

✍ Scribed by Gisela Kramer; Wieslaw Kudlicki; Diane McCarthy; Tamara Tsalkova; Donald Simmons; Boyd Hardesty

Book ID: 115539217
Publisher: Elsevier Science
Year: 1999
Tongue: English
Weight: 196 KB
Volume: 31
Category: Article
ISSN: 1357-2725
DOI: 10.1016/s1357-2725(98)00143-5

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## Abstract __Deactivation of release factor 1 by polyclonal antibodies in an in vitro translation system, which was used to express the esterase gene, led to the reversible elimination of naturally occurring termination. This technique allowed the antibiotic puromycin to be used as an acceptor sub