n-Butyrate induces plasminogen activator inhibitor type 1 messenger RNA in cultured Hep G2 cells

✍ Scribed by T J Smith; J J Piscatelli; V Andersen; H Wang; P Lance

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 398 KB
• Volume: 23
• Category: Article
• ISSN: 0270-9139
• DOI: 10.1002/hep.510230430

✦ Synopsis

n-Butyrate, a short-chain aliphatic carboxylic acid n-Butyrate, a four-carbon short-chain aliphatic carwith pleiotropic actions, is present at high concentraboxylic acid, and compounds structurally related to it tions in the portal circulation and thus may play an imexert numerous actions on cells in culture. Regulation portant role in the regulation of specific gene expression of cellular differentiation, proliferation, receptor abunin the mammalian liver. We report here that n-butyrate dance, specific gene expression, and the activities of can increase substantially the level of plasminogen actiseveral key enzymes are among the pleiotropic effects vator inhibitor type 1 (PAI-1) messenger RNA (mRNA) observed with n-butyrate treatment. [1][2][3][4][5][6][7][8] Although these in Hep G2 cells, up to eightfold above control cultures.

activities are well documented in vitro, whether they

Maximal effects occurred at a concentration of 3 mmol/L occur in the whole body remains uncertain. Human n-butyrate and with a treatment period of 8 to 12 hours. tissues exposed to extremely high (mmol/L range) con-Increases in PAI-1 mRNA were accompanied by modest increases (twofold) in the encoded protein as assessed centrations of short-chain fatty acids (SCFA) include by specific enzyme-linked immunosorbent assay and by the colon and liver. 9,10 Cells derived from these tissues [ 35 S]methionine incorporation into immunoprecipitable and maintained in culture should represent potentially PAI-1 in the culture medium. Nuclear run-on studies good models for assessing the impact of high SCFA showed that the rate of transcription of the PAI-1 gene concentrations on cellular metabolism.