Mutator mutants of Escherichia coli carrying a defect in the DNA polymerase III τ subunit
✍ Scribed by Phuong T. Pham; Wei Zhao; Roel M. Schaaper
- Book ID
- 110832820
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 559 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0950-382X
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The subunit of DNA polymerase III, the main replicative polymerase of Escherichia coli, has been examined by circular dichroism and by NMR spectroscopy. The polymerase core consists of three subunits: ␣, ⑀, and , with ␣ possessing the polymerase activity, ⑀ functioning as a proofreading exonuclease,
A mutant of Escherichia coli K12 is described in which sigma and alpha subunits of the DNA-dependent RNA polymerase (EC 2.7.7.6) are produced at the rates much higher than in the normal strain. The rate of synthesis for sigma subunit was found to be at least 10-times higher, though the rapid degrada