Mutations affecting translational fidelity in the eucaryote Podospora anserina: Characterization of two ribosomal restrictive mutations

Cold-sensitivity of a double mutant stra

Cold-sensitivity of a double mutant strain combining two ribosomal mutations in the ascomycete Podospora anserina

✍ Crouzet, M. ;Bégueret, J. 📂 Article 📅 1978 🏛 Springer 🌐 English ⚖ 457 KB

A double mutant strain combining two ribosomal mutations conferring resistance to cycloheximide exhibits a cold-sensitive phenotype. At low temperature the biosynthesis of the 60S subunit is impaired. Genetic analysis of cold-resistant revertants have shown that this double mutant strain can be used