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Cold-sensitivity of a double mutant strain combining two ribosomal mutations in the ascomycete Podospora anserina

✍ Scribed by Crouzet, M. ;Bégueret, J.


Publisher
Springer
Year
1978
Tongue
English
Weight
457 KB
Volume
165
Category
Article
ISSN
0026-8925

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✦ Synopsis


A double mutant strain combining two ribosomal mutations conferring resistance to cycloheximide exhibits a cold-sensitive phenotype. At low temperature the biosynthesis of the 60S subunit is impaired. Genetic analysis of cold-resistant revertants have shown that this double mutant strain can be used efficiently to isolate new ribosomal mutations.