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Mutations affecting RNA Polymerase associated with Rifampicin Resistance in Escherichia coli

โœ Scribed by EZEKIEL, DAVID H.; HUTCHINS, JOAN E.


Book ID
120438419
Publisher
Nature Publishing Group
Year
1968
Tongue
English
Weight
179 KB
Volume
220
Category
Article
ISSN
0028-0836

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RNA polymerase rifampicin resistance mut
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Five recombinant plasmids, pBK2646, pBK611, pRC3, pRC4 and pRC5, carrying rpoB rifampicin-resistant RNA-polymerase genes were obtained. The sequence analysis of these plasmids revealed certain structural changes in the rpoB gene which specify corresponding alterations in the beta-subunit of RNA poly

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The unusual recombinant plasmid pRC19 carrying the N-terminal fragment of the Escherichia coli RNA polymerase rpoB gene was found to specify high level rifampicin resistance of E. coli cells. Sequence analysis of this plasmid revealed one substitution only: transversion G----T, leading to amino acid