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RNA polymerase rifampicin resistance mutations in Escherichia coli: Sequence changes and dominance

✍ Scribed by Ovchinnikov, Yuri A. ;Monastyrskaya, Galina S. ;Guriev, Sergei O. ;Kalinina, Nadezhda F. ;Sverdlov, Eugene D. ;Gragerov, Alexander I. ;Bass, Irina A. ;Kiver, Irina F. ;Moiseyeva, Elena P. ;Igumnov, Vladimir N. ;Mindlin, Sofia Z. ;Nikiforov, Vadim G. ;Khesin, Roman B.

Publisher: Springer
Year: 1983
Tongue: English
Weight: 552 KB
Volume: 190
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00330662

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✦ Synopsis

Five recombinant plasmids, pBK2646, pBK611, pRC3, pRC4 and pRC5, carrying rpoB rifampicin-resistant RNA-polymerase genes were obtained. The sequence analysis of these plasmids revealed certain structural changes in the rpoB gene which specify corresponding alterations in the beta-subunit of RNA polymerase. Some functional properties of the corresponding mutant strains and their RNA polymerases have been investigated.

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