Mutational Specificity of the Ferrous Ion in a supF Gene of Escherichia coli
โ Scribed by S. Akasaka; K. Yamamoto
- Book ID
- 115577026
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 240 KB
- Volume
- 213
- Category
- Article
- ISSN
- 0006-291X
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๐ SIMILAR VOLUMES
Forward mutations induced by 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU) in the supF gene of Escherichia coli were recovered from bacteria deficient in nucleotide excision repair and in DNA-alkyltransferase activity. Bacteria were exposed to 0.4 mM CCNU (in vivo supF mutagenesis), increasing
Escherichia coli K12 strain KS40 and plasmid pKY241 were designed for easy screening of supF mutations in plasmid pZ189. KS40 is a nalidixic acid-resistant (gyrA) derivative of MBM7070 (lacZ(am)CA7020). Using in vitro mutagenesis, an amber mutation was introduced into the cloned gyrA structural gene