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An Escherichia coli topB mutant increases deletion and frameshift mutations in the supF target gene

โœ Scribed by Norio Uematsu; Sachiko Eda; Kazuo Yamamoto


Book ID
117614514
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
218 KB
Volume
383
Category
Article
ISSN
0921-8777

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Escherichia coli K12 strain KS40 and plasmid pKY241 were designed for easy screening of supF mutations in plasmid pZ189. KS40 is a nalidixic acid-resistant (gyrA) derivative of MBM7070 (lacZ(am)CA7020). Using in vitro mutagenesis, an amber mutation was introduced into the cloned gyrA structural gene