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Mutational specificity: Database and software for the analysis of mutations at the lacl gene in both transgenic rodents and bacteria

✍ Scribed by Neal F. Cariello; Nancy J. Gorelick

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 773 KB
Volume: 28
Category: Article
ISSN: 0893-6692
DOI: 10.1002/(sici)1098-2280(1996)28:4<397::aid-em14>3.0.co;2-a

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✦ Synopsis

The use of transgenic rodents for the study of genetic toxicology has increased dramatically in the past several years. A great deal of the recent work has employed the lac/ locus in transgenic mice. In addition to the transgenic data, a substantial amount of information exists regarding mutation of the lac/ gene in bacteria. In an effort to centralize the informotion regarding mutations in the laclgene in both rodents and bacteria, we have created a computerized database that contains information about DNA sequence alterations on about 500 mutations in transgenic rodents and 8,000 mutations in bacteria. We have also produced a software package for the analysis of the locl database. Routines have been developed for the analysis of single base sub stitutions, including programs to (i) determine if two mutational spectra are different; (ii) determine if mutations show a DNA strand bias; (iii) determine the frequency of transitions and transversions; (iv) display the number and kind of mutations observed at each base in the coding region; (v) perform nearest neighbor analysis; and (vi) display mutable amino acids in the lac/ protein. The s o b a r e runs only on IBMcompatible machines running Microsoft Windows. The software and lac/ database are freely available via the Internet (

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