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Mutational analysis of the n-terminus in Schistocerca gregaria ion-transport peptide expressed in Drosophila Kc1 cells

โœ Scribed by Y. Zhao; J. Meredith; H.W. Brock; J.E. Phillips


Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
218 KB
Volume
58
Category
Article
ISSN
0739-4462

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We have produced an active form of Schistocerca gregaria ion transport peptide (ITP) in an insect cell expression system. Transformed Drosophila Kc1 cells secreted a form of ITP into the cell culture medium that was proteolytically cleaved correctly at the amino (N)-terminus. Concentrated culture su