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Analysis of an insect neuropeptide, Schistocerca gregaria ion transport peptide (ITP), expressed in insect cell systems

โœ Scribed by T. A. Pfeifer; D. Hegedus; Y.-J. Wang; Y. Zhao; J. Meredith; H. W. Brock; J. E. Phillips; T. A. Grigliatti; D. A. Theilmann

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
131 KB
Volume
46
Category
Article
ISSN
0739-4462

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๐Ÿ“œ SIMILAR VOLUMES

Analysis of an insect neuropeptide, Schi
Analysis of an insect neuropeptide, Schistocerca gregaria ion transport peptide (ITP), expressed in insect cell systems
โœ T.A. Pfeifer; D. Hegedus; Y.-J. Wang; Y. Zhao; J. Meredith; H. W. Brock; J. E. P ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 166 KB

We have produced an active form of Schistocerca gregaria ion transport peptide (ITP) in an insect cell expression system. Transformed Drosophila Kc1 cells secreted a form of ITP into the cell culture medium that was proteolytically cleaved correctly at the amino (N)-terminus. Concentrated culture su

Characterization of monovalent ion trans
Characterization of monovalent ion transport systems in an insect cell line (Manduca sexta embryonic cell line che)
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The monovalent ion transport systems of an immortalized insect cell line (CHE) have been investigated. These cells are unusual in that unlike most vertebrate cells, their normal extracellular environment consists of high potassium and low sodium concentrations. CHE cells maintained high intracellula