Mutation scanning of the CFTR gene by high-resolution melting analysis (HRM)
β Scribed by P. Krenkova; A. Stambergova; P. Norambuena; M. Macek Jr.
- Book ID
- 118641454
- Publisher
- Elsevier Science
- Year
- 2008
- Tongue
- English
- Weight
- 47 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1569-1993
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Genetic analysis of BRCA1 by sequencing is often preceded by a scanning method like denaturing gradient gel electrophoresis (DGGE), protein truncation test (PTT) or DHPLC. High-resolution melting curve (HRM) analysis is a promising and economical method for high-throughput mutation scanning. The Eur
Mutation scanning using high-resolution melting curve analysis (HR-melt) is an effective and sensitive method to detect sequence variations. However, the presence of a common SNP within a mutation scanning amplicon may considerably complicate the interpretation of results and increase the number of