✦ LIBER ✦

Mutation in the TP53 gene in colorectal carcinoma detected by polymerase chain reaction

✍ Scribed by Eun-Soo Han; Mary Pat Moyer; Susan Naylor; Dr. Alan Y. Sakaguchi

Book ID: 102844525
Publisher: John Wiley and Sons
Year: 1991
Tongue: English
Weight: 558 KB
Volume: 3
Category: Article
ISSN: 1045-2257
DOI: 10.1002/gcc.2870030411

No coin nor oath required. For personal study only.

✦ Synopsis

The human TP53 gene is a possible tumor suppressor since TP53 gene mutations are observed in >70% of sporadic colorectal carcinoma DNAs. In genomic DNAs from seven colon cancer cell samples, a 405 base pair DNA fragment containing exon 5, intron 5, and exon 6 of the Tf53 gene was amplified by polymerase chain reaction and analyzed for mutations. One sample [human colon cancer (HCC) 2781 was found to have a TP53 mutation altering the amino acid glutamine 167 in exon 5. A deletion of 2 bases changed glutamine I67 (CAG) to alanine (GCA) and the resulting frame-shift produced an in-frame stop codon at amino acid 179. While the normal TP53 gene gives rise to a 53 kD protein, the estimated size of this mutant TP53 protein if expressed would be approximately 20 kD.

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