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Mutants of CHO cells resistant to the protein synthesis inhibitor emetine: Genetic and biochemical characterization of second-step mutants

✍ Scribed by Radhey S. Gupta; Louis Siminovitch


Book ID
112559747
Publisher
Springer
Year
1978
Tongue
English
Weight
944 KB
Volume
4
Category
Article
ISSN
1572-9931

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Mutants resistant to 200 micrograms/ml of the alkaloid lycorine (LYCR) in non-fermentable substrate were isolated after nitrosoguanidine mutagenesis. Tetrad analysis and growth of heterozygous (LYCR/lycS) diploids from two different mutants revealed that a single nuclear and dominant mutation is res