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Mutant of E. coli containing an Altered DNA-dependent RNA Polymerase

✍ Scribed by TOCCHINI-VALENTINI, G. P.; MARINO, P.; COLVILL, A. J.


Book ID
120438418
Publisher
Nature Publishing Group
Year
1968
Tongue
English
Weight
101 KB
Volume
220
Category
Article
ISSN
0028-0836

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## Abstract __E. Coli__ RNA polymerase holoenzyme consists of two large subunitsβ€”Ξ² and Ξ²' (molecular weights 155,000 and 165,000), of two identical Ξ± subunits (molecular weight 36,500), and of the initiation factor Ξ± (molecular weight 86,000). The complete amino acid sequence of the RNA polymerase

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We have analyzed some chemical properties of the sigma subunit of RNA polymerase from the sigma mutants: rpoD1 (Gross et al., 1978), rpoD2 (formerly known as alt-1) (Silverstone et al., 1972; Travers et al., 1978), and rpoD800 (Gross et al., 1979). Each of the three mutants is located at about 66 mi