Mutant of E. coli containing an Altered DNA-dependent RNA Polymerase
β Scribed by TOCCHINI-VALENTINI, G. P.; MARINO, P.; COLVILL, A. J.
- Book ID
- 120438418
- Publisher
- Nature Publishing Group
- Year
- 1968
- Tongue
- English
- Weight
- 101 KB
- Volume
- 220
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/220275a0
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## Abstract __E. Coli__ RNA polymerase holoenzyme consists of two large subunitsβΞ² and Ξ²' (molecular weights 155,000 and 165,000), of two identical Ξ± subunits (molecular weight 36,500), and of the initiation factor Ξ± (molecular weight 86,000). The complete amino acid sequence of the RNA polymerase
We have analyzed some chemical properties of the sigma subunit of RNA polymerase from the sigma mutants: rpoD1 (Gross et al., 1978), rpoD2 (formerly known as alt-1) (Silverstone et al., 1972; Travers et al., 1978), and rpoD800 (Gross et al., 1979). Each of the three mutants is located at about 66 mi