𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Mutagenicity of γ-radiation, mitomycin C, and etoposide in the Hprt and Tk genes of Tk+/− mice

✍ Scribed by Vasily N. Dobrovolsky; Joseph G. Shaddock; Robert H. Heflich

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
117 KB
Volume
39
Category
Article
ISSN
0893-6692

No coin nor oath required. For personal study only.

✦ Synopsis

The recently developed Tk(+/-) mouse detects in vivo somatic cell mutation in the endogenous, autosomal Tk gene. To evaluate the sensitivity of this model, we have treated Tk(+/-) mice with three agents that induce DNA damage by different mechanisms, and determined spleen lymphocyte mutant frequencies (MFs) in the autosomal Tk gene and in the X-linked Hprt gene. gamma-Radiation, which produces single- and double-strand breaks by nonspecific oxidative stress, efficiently increased Hprt MF, but not Tk MF. Mitomycin C, which produces bulky DNA monoadducts and crosslinks, was mutagenic in both the Hprt and Tk genes, but the response was greater in the Tk gene. An inhibitor of the ligase function of DNA topoisomerase II, etoposide, did not increase Hprt MF, and induced a small, but nonsignificant increase in Tk MF. Combined with previous data, the results indicate that the two genes are differentially sensitive to many agents, and that the Tk gene is more sensitive than the Hprt gene to some, but not all types of DNA damage.

📜 SIMILAR VOLUMES

Relative mutagenic potencies of several
Relative mutagenic potencies of several nucleoside analogs, alone or in drug pairs, at the HPRT and TK loci of human TK6 lymphoblastoid cells
✍ Meghan M. Carter; Salina M. Torres; Dennis L. Cook Jr.; Consuelo L. McCash; Mia 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 189 KB

## Abstract Experiments were performed to investigate the impact of didanosine (ddI), lamivudine (3TC), and stavudine (d4T) on cell survival and mutagenicity in two reporter genes, hypoxanthine‐guanine phosphoribosyltransferase (__HPRT__) and thymidine kinase (__TK__), using a cell cloning assay fo

Nitric oxide–induced mutations in the HP
Nitric oxide–induced mutations in the HPRT gene of human lymphoblastoid TK6 cells and in Salmonella typhimurium
✍ John C. Zhuang; Teresa L. Wright; Teresa deRojas-Walker; Steven R. Tannenbaum; G 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 84 KB 👁 1 views

Characterization of mutations induced by NO in different experimental systems will facilitate elucidation of mechanisms underlying its genotoxicity. The mutagenic specificity of NO in human cells is of particular interest in view of its potential role in inflammation-associated carcinogenesis. We co

Molecular analysis of in vivo mutations
Molecular analysis of in vivo mutations induced by N-ethyl-N-nitrosourea in the autosomal Tk and the X-linked Hprt genes of mouse lymphocytes
✍ Vasily N. Dobrovolsky; Tao Chen; Robert H. Heflich 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 146 KB 👁 2 views

The endogenous, autosomal Tk gene is a potentially useful reporter of in vivo mutation since it may recover a wider range of mutational events than the X-linked Hprt gene or bacterial transgenes. In this study, we characterized mutations produced in the Tk gene of Tk ϩ/Ϫ mice and compared them with

System Issues: Mutagenic response of the
System Issues: Mutagenic response of the endogenous hprt gene and lacl transgene in benzo[a]pyrene-treated Big Blue™ B6C3F1 mice
✍ Thomas R. Skopek; Kristy L. Kort; Deborah R. Marino; Lakshmi V. Mittal; Diane R. 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 807 KB

Big Blue@ (BB) and generic B6C3F1 mice were given one to three i.p. injections of 50 mg/kg benzo[a]pyrene (B[a]P) in DMSO every other day to achieve cumulative doses of 50 to 150 mg/kg. Three weeks after treatment, the mutation frequency at the endogenous hprt gene and lac/ transgene was measured in