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Mutagenesis of Acetobacter methanolicus MB58 with the transposon Tn5

✍ Scribed by P. Dobrowolski; Dr. M. W. Gründig

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
148 KB
Volume
30
Category
Article
ISSN
0233-111X

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✦ Synopsis

Transposon mutagenesis was applied to the isolation of mutants of the facultatively methylotrophic Acetobacter methanolzcus MB58. The transposon Tn5 (pSU2011) was transferred from Escherichiu eoli SM 10 by means of conjugation to Acetobacter methanolicus MB58. Four out of 1850 stable Km-resistant transconjugants were identified that were formaldehyde sensitive and failed to grow on methanol.

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