Influence of cytochrome c-deficiency on the energy conservation of whole cells of Acetobacter methanolicus sp. MB 58
✍ Scribed by Dr. N. Loffhagen; W. Babel
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 403 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0233-111X
No coin nor oath required. For personal study only.
✦ Synopsis
The ability of A . melhunolicus sp. MB 58 and of a cytochrome c-deficient mutant ( A . methanolicus sp. MB 58105) to synthesize ATP by the oxidation of methanol, ethanol, glucose, formate and formaldehyde was investigated. It was found out that cellsof the wild type were energized and that cells of the mutant were not energized by the oxidation of ethanol.
The energization of cells by the oxidation ofglucose, formate and formaldehyde is obviously not influenced by the cytochrome c defect. The results point to a coupling of the alcohol dehydrogenase at cytochrome c, as already postulated in a previous paper for the alcohol and the methanol dehydrogenases of A . merhnolicus sp. MB 70.
The dehydrogenases responsible for the oxidation of glucose and formate should be coupled to other cytochromes of the respiratory chain. Evidence for the coupling of the formaldehyde dehydrogenase could not be obtained, because it was not possible to differentiate between cytochromedependent oxidation of formaldehyde and the NAD'dependent oxidations after fixation of formaldehyde.