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Mutagenesis in Escherichia coli

✍ Scribed by Bridges, B. A. ;Law, J. ;Munson, R. J.


Publisher
Springer
Year
1968
Tongue
English
Weight
537 KB
Volume
103
Category
Article
ISSN
0026-8925

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Site-specific mutagenesis of dihydrofola
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Two site-specific mutations of dihydrofolate reductase from Escherichiu coli based on the x-ray crystallographic structure were constructed. The first mutation (His-45 + Gln) is aimed at assessing the interaction between the imidazole moiety and the pyrophosphate backbone of NADPH. The second (Thr-1